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Glentham Life Sciences 4-thiouridine 4su
4 Thiouridine 4su, supplied by Glentham Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4-thiouridine+4su/4su+gn6085/pm40447771-302-9-11
Average 90 stars, based on 1 article reviews
4-thiouridine 4su - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Sequencing:

Article Title: The PNUTS phosphatase complex controls transcription pause release.
Article Snippet: In order to compare the effect of labelling time, a separate cTT-seq experiment was conducted in the PNUTS degron cell line in which 1x108 ESCs were labelled with 500 mM 4-thiouridine (4sU, Glentham Life Sciences) for 5 min and harvested in TRIzol.

Article Title: A CpG island-encoded mechanism protects genes from premature transcription termination
Article Snippet: In brief, 9 million ESCs and 3 million Drosophila SG4 cells were labelled with 500 μM 4-thiouridine (4sU, Glentham Life Sciences) for 15 min and harvested into TRIzol reagent.

Article Title: The PNUTS phosphatase complex controls transcription pause release.
Article Snippet: 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted and depleted from any gDNA carry-over using the Direct-zol RNAMiniprep Plus kit (R2072 Zymo Research) as per themanufacturer’s protocol.

Article Title: PAF1C-mediated activation of CDK12/13 kinase activity is critical for CTD phosphorylation and transcript elongation.
Article Snippet: Nascent RNA was then labelled using a 15-minute pulse of 4-thiouridine (4sU; Glentham Life Sciences, #GN6085), after which the reaction was stopped by aspiration of media and addition of 1mL Trizol (Thermo Fisher Scientific, #15596026).

Article Title: Transcriptional repression facilitates RNA:DNA hybrid accumulation at DNA double-strand breaks.
Article Snippet: For DRB/TTchem-seq, DSBs were induced by treating DIvA cells with 300 nM OHT for 3 h, followed by a 1 h treatment with 100 μM DRB (Sigma-Aldrich, D1916).



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KEY RESOURCES TABLE
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KEY RESOURCES TABLE

Journal: Cell reports

Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms

doi: 10.1016/j.celrep.2024.115113

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 4-Thiouridine (4sU) , Millipore Sigma , T4509.

Techniques: Synthesized, Virus, Plasmid Preparation, shRNA, Recombinant, Transfection, Injection, Software, Imaging